Study on Chemical Constituents in Ferula feruloides

To study the chemical constituents of Ferula. ferulaeoides (Steud.) Korov. and their antitumor activities, it's chemical constituents were separated and purified by sephadex LH-20, silica gel, semi-preparative high performance liquid chromatography (HPLC) methods, and their structures were identified by physicochemical property and NMR, HRMS spectroscopic methods. The absolute configuration of new compound was determined by ECD. The cytotoxic activity of isolated compounds on human breast cancer (MCF-7) cell line was determined by MTT assay. Fifteen compounds ( 1 - 15 ) were isolated, and were identified as 1- (2- hydroxyl- 4- methoxybenzoyl)- 2S*- hydroxy- 5,9,13- trimethyl- 4(E),8(E),12- tetradecatrien- 1- one ( 1 ), guaiol ( 2 ), 1- (2,4- dihydroxyphenyl)- 3,7,11- trimethyl- 3- vinyl- 6(E),10- dodecadiene- 1- one ( 3 )，dshamirone ( 4 ), 2,3- dihydro-7- hydroxy- 2S^*,3R^*- dimethyl- 2- 4,8-dimethyl- 3(E),7- nonadienyl- furo3,2-ccoumarin ( 5 ),3- (2-hydroxyl-4-methoxybenzoyl)- 4S^*,5R^*- dimethyl- 5- 4,8- dimethyl- 3(E),7(E)- nonadien- 1- yltetrahydro- 2- furanone ( 6 )，fukanedone A ( 7 ), 2,3- dihydro- 7- methoxy- 2S^*,3R^*- dimethyl- 2- 4- methyl- 5- (4-methyl-2-furyl)- 3(E)- pentenyl- furo3,2-ccoumarin ( 8 ), umbelliprenin ( 9 ), 2,3- dihydro- 7- methoxy- 2S^*,3S^*- dimethyl- 3- 4,8- dimethyl- 3(E),7- nonadienyl- furo3,2-ccoumarin ( 10 ), 2,3- dihydro- 7- methoxy- 2S^*,3R^*- dimethyl- 3- 4,8-dimethyl-3(E),7-nonadienyl- furo3,2-ccoumarin ( 11 ), 2,3- dihydro- 7- hydroxy- 2S^*,3R^*- dimethyl- 3- 4,8- dimethyl- 3(E),7- nonadienyl- furo3,2-ccoumarin ( 12 ), 2,3- dihydro- 7- methoxy- 2S^*,3R^*- dimethyl- 2- 4,8- dimethyl- 3(E),7- nonadienyl- furo3,2-ccoumarin ( 13 ),2,3- dihydro- 7- methoxy- 2R^*,3R^*- dimethyl- 2- 4,8- dimethyl- 3(E),7- nonadienyl- furo3,2-ccoumarin ( 14 ), ferulaeone C ( 15 ). Compound 1 is a new compound, compound 9 is isolated from this plant for the first time. Compounds 3 、 4 、 5 and 15 show potential antitumor activity with 50% inhibitory concentration ( IC₅₀) values of 15.11, 12.98, 18.01 and 12.87 μmol/L, respectively.