A Study on the Effect of Growth of Endothelial Cells upon Biological Modifications of Silk

Immobilized with silk solution, staphylococcal protein A (SPA) can be made into an insoluble silk membrane and then adsorbed to antiRGD antibody IgG to an SPA silk membrane, then adsorbed RGD (synthetic peptide, GLY-ARG-GLY-ASP-SER-PRO-LYS) which is adhesive and growth factor to IgG-SPA silk membrane(namely RGD-IgG-SPA silk membrane). The activity of SPA immobilized silk membrane was investigated by the enzyme-linked immunosorbent assay(ELISA). The results demonstrated that the stability of SPA immobilized silk membrane has been greatly improved. Vascular endothelial cells were seeded onto the modified silk membrane. Effectiveness of the absorbance in the survival of cells was verified by tetrazolium-based assay (MTT). It demonstrated that RGD adsorbed IgG-SPA immobilized silk membrane could keep its bioactivity functionally.