Advanced microfluidic systems with integrated microstructures for enhanced exosome isolation and analysis

Liyuan Guo; Hang Li; Menglei Zhao; Donghui Li; Zhiming Jiang; Bin Fu; Chao Yang; Jiangjiang Zhang; Kangfu Chen; Rongxin Fu; Huikai Xie; Shengyuan Deng; Shuailong Zhang

doi:10.1016/j.trac.2026.118881

Advanced microfluidic systems with integrated microstructures for enhanced exosome isolation and analysis

Liyuan Guo
, Hang Li^*
, Menglei Zhao
, Donghui Li
, Zhiming Jiang
, Bin Fu
, Chao Yang
, Jiangjiang Zhang
, Kangfu Chen
, Rongxin Fu
, Huikai Xie
, Shengyuan Deng^*
, Shuailong Zhang^*

^*此作品的通讯作者

Beijing Institute of Technology
National Center for Protein Sciences (Beijing)
Nanjing University of Science and Technology

科研成果: 期刊稿件 › 文献综述 › 同行评审

摘要

Exosomes, as extracellular vesicles involved in intercellular communication, are emerging as important biomarkers for liquid biopsy and disease monitoring. Conventional methods for isolating exosomes, such as ultracentrifugation and polymer precipitation, have shown limitations of low efficiency, lengthy processing, and poor scalability. To address these challenges, microfluidics integrated with microstructures has gained prominence as a high-throughput, automated alternative. This review discusses recent advances in these integrated platforms, focusing on the synergistic mechanisms of physical confinement and chemical affinity for enhanced exosome capture. We systematically categorize isolation strategies into immunoaffinity-based, size-based approaches, and hybrid strategies, evaluating their performance in complex biological matrices. Additionally, we explore the integration of these systems with advanced biosensing or analytical technologies, highlighting their transformative potential for precise, scalable and clinically viable exosome-based diagnostics and therapeutics.

源语言	英语
文章编号	118881
期刊	TrAC - Trends in Analytical Chemistry
卷	201
DOI	https://doi.org/10.1016/j.trac.2026.118881
出版状态	已出版 - 8月 2026

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title = "Advanced microfluidic systems with integrated microstructures for enhanced exosome isolation and analysis",

abstract = "Exosomes, as extracellular vesicles involved in intercellular communication, are emerging as important biomarkers for liquid biopsy and disease monitoring. Conventional methods for isolating exosomes, such as ultracentrifugation and polymer precipitation, have shown limitations of low efficiency, lengthy processing, and poor scalability. To address these challenges, microfluidics integrated with microstructures has gained prominence as a high-throughput, automated alternative. This review discusses recent advances in these integrated platforms, focusing on the synergistic mechanisms of physical confinement and chemical affinity for enhanced exosome capture. We systematically categorize isolation strategies into immunoaffinity-based, size-based approaches, and hybrid strategies, evaluating their performance in complex biological matrices. Additionally, we explore the integration of these systems with advanced biosensing or analytical technologies, highlighting their transformative potential for precise, scalable and clinically viable exosome-based diagnostics and therapeutics.",

keywords = "Artificial intelligence, Exosome enrichment, Microfluidics, Microstructures",

author = "Liyuan Guo and Hang Li and Menglei Zhao and Donghui Li and Zhiming Jiang and Bin Fu and Chao Yang and Jiangjiang Zhang and Kangfu Chen and Rongxin Fu and Huikai Xie and Shengyuan Deng and Shuailong Zhang",

note = "Publisher Copyright: {\textcopyright} 2026 Elsevier B.V.",

year = "2026",

month = aug,

doi = "10.1016/j.trac.2026.118881",

language = "English",

volume = "201",

journal = "TrAC - Trends in Analytical Chemistry",

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publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Advanced microfluidic systems with integrated microstructures for enhanced exosome isolation and analysis

AU - Guo, Liyuan

AU - Li, Hang

AU - Zhao, Menglei

AU - Li, Donghui

AU - Jiang, Zhiming

AU - Fu, Bin

AU - Yang, Chao

AU - Zhang, Jiangjiang

AU - Chen, Kangfu

AU - Fu, Rongxin

AU - Xie, Huikai

AU - Deng, Shengyuan

AU - Zhang, Shuailong

PY - 2026/8

Y1 - 2026/8

N2 - Exosomes, as extracellular vesicles involved in intercellular communication, are emerging as important biomarkers for liquid biopsy and disease monitoring. Conventional methods for isolating exosomes, such as ultracentrifugation and polymer precipitation, have shown limitations of low efficiency, lengthy processing, and poor scalability. To address these challenges, microfluidics integrated with microstructures has gained prominence as a high-throughput, automated alternative. This review discusses recent advances in these integrated platforms, focusing on the synergistic mechanisms of physical confinement and chemical affinity for enhanced exosome capture. We systematically categorize isolation strategies into immunoaffinity-based, size-based approaches, and hybrid strategies, evaluating their performance in complex biological matrices. Additionally, we explore the integration of these systems with advanced biosensing or analytical technologies, highlighting their transformative potential for precise, scalable and clinically viable exosome-based diagnostics and therapeutics.

AB - Exosomes, as extracellular vesicles involved in intercellular communication, are emerging as important biomarkers for liquid biopsy and disease monitoring. Conventional methods for isolating exosomes, such as ultracentrifugation and polymer precipitation, have shown limitations of low efficiency, lengthy processing, and poor scalability. To address these challenges, microfluidics integrated with microstructures has gained prominence as a high-throughput, automated alternative. This review discusses recent advances in these integrated platforms, focusing on the synergistic mechanisms of physical confinement and chemical affinity for enhanced exosome capture. We systematically categorize isolation strategies into immunoaffinity-based, size-based approaches, and hybrid strategies, evaluating their performance in complex biological matrices. Additionally, we explore the integration of these systems with advanced biosensing or analytical technologies, highlighting their transformative potential for precise, scalable and clinically viable exosome-based diagnostics and therapeutics.

KW - Artificial intelligence

KW - Exosome enrichment

KW - Microfluidics

KW - Microstructures

UR - https://www.scopus.com/pages/publications/105036566342

U2 - 10.1016/j.trac.2026.118881

DO - 10.1016/j.trac.2026.118881

M3 - Review article

AN - SCOPUS:105036566342

SN - 0165-9936

VL - 201

JO - TrAC - Trends in Analytical Chemistry

JF - TrAC - Trends in Analytical Chemistry

M1 - 118881

ER -

Advanced microfluidic systems with integrated microstructures for enhanced exosome isolation and analysis

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